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Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA.
DNA complementary to mRNA of human immunoglobulin E heavy chain (epsilon chain) isolated and purified from U266 cells has been synthesized and inserted into the PstI site of pBR322 by G-C tailing. This recombinant plasmid was used to transform E. coli chi 1776 to screen 1445 tetracycline resistant c...
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Main Authors: | , , , , , , , , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
1983
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Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325748/ https://ncbi.nlm.nih.gov/pubmed/6300763 |
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