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Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.

A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene. An appropriate cloning site...

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Detalles Bibliográficos
Main Authors: Gray, M R, Colot, H V, Guarente, L, Rosbash, M
Formato: Artigo
Idioma:Inglês
Publicado: 1982
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC347175/
https://ncbi.nlm.nih.gov/pubmed/6815653
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