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Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.
A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene. An appropriate cloning site...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347175/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6815653/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.21.6598 |
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