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Isolation and Primary Culture of Rat Hepatic Cells

Primary hepatocyte culture is a valuable tool that has been extensively used in basic research of liver function, disease, pathophysiology, pharmacology and other related subjects. The method based on two-step collagenase perfusion for isolation of intact hepatocytes was first introduced by Berry an...

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Detalhes bibliográficos
Main Authors: Shen, Ling, Hillebrand, Allix, Wang, David Q.-H., Liu, Min
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3471302/
https://ncbi.nlm.nih.gov/pubmed/22781923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3917
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