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Synthesis of beta-hexosaminidase in cell-free translation and in intact fibroblasts: an insoluble precursor alpha chain in a rare form of Tay-Sachs disease.

RNA was isolated from human term placenta or cultured fibroblasts and translated in a rabbit reticulocyte system in the presence of [35S]methionine; the translation products were immunoprecipitated with antisera made against beta-hexosaminidase or its isolated alpha and beta chains and analyzed by p...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Proia, R L, Neufeld, E F
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1982
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347121/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6959123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.20.6360
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