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Synthesis of beta-hexosaminidase in cell-free translation and in intact fibroblasts: an insoluble precursor alpha chain in a rare form of Tay-Sachs disease.
RNA was isolated from human term placenta or cultured fibroblasts and translated in a rabbit reticulocyte system in the presence of [35S]methionine; the translation products were immunoprecipitated with antisera made against beta-hexosaminidase or its isolated alpha and beta chains and analyzed by p...
Gespeichert in:
| Veröffentlicht in: | Proc Natl Acad Sci U S A |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1982
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347121/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6959123/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.20.6360 |
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