An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.

We describe the synthesis, cloning, expression, and in vivo function of a suppressor tRNA gene in mammalian cells. By using "primer-directed mutagenesis" on a Xenopus laevis tyrosine tRNA gene cloned into the recombinant single-strand phage M13mp5, we have generated an amber suppressor tRNA gene tha...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Laski, F A, Belagaje, R, RajBhandary, U L, Sharp, P A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1982
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347000/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310546/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.19.5813
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