An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.
We describe the synthesis, cloning, expression, and in vivo function of a suppressor tRNA gene in mammalian cells. By using "primer-directed mutagenesis" on a Xenopus laevis tyrosine tRNA gene cloned into the recombinant single-strand phage M13mp5, we have generated an amber suppressor tRNA gene tha...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1982
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347000/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310546/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.19.5813 |
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