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Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays
We describe a method to screen pools of DNA from multiple transposon lines for insertions in many genes simultaneously. We use thermal asymmetric interlaced–PCR, a hemispecific PCR amplification protocol that combines nested, insertion-specific primers with degenerate primers, to amplify DNA flankin...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The National Academy of Sciences
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC34684/ https://ncbi.nlm.nih.gov/pubmed/11416215 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.121189598 |
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