Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays

We describe a method to screen pools of DNA from multiple transposon lines for insertions in many genes simultaneously. We use thermal asymmetric interlaced–PCR, a hemispecific PCR amplification protocol that combines nested, insertion-specific primers with degenerate primers, to amplify DNA flankin...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Mahalingam, Ramamurthy, Fedoroff, Nina
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2001
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC34684/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11416215/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.121189598
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