Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.
A novel method, not relying on genetic complementation of a mutation, was used to clone a gene for translational initiation factor IF2. Two clones from a cosmid library of total Escherichia coli DNA were isolated for their ability to overproduce IF2 in vivo as determined by quantitative immunoblotti...
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| Опубліковано в:: | Proc Natl Acad Sci U S A |
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| Автори: | , , , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
National Academy of Sciences
1982
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346821/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6214789/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.16.5033 |
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