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Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.

A novel method, not relying on genetic complementation of a mutation, was used to clone a gene for translational initiation factor IF2. Two clones from a cosmid library of total Escherichia coli DNA were isolated for their ability to overproduce IF2 in vivo as determined by quantitative immunoblotti...

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Autors principals: Plumbridge, J A, Howe, J G, Springer, M, Touati-Schwartz, D, Hershey, J W, Grunberg-Manago, M
Format: Artigo
Idioma:Inglês
Publicat: 1982
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC346821/
https://ncbi.nlm.nih.gov/pubmed/6214789
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