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Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.

A novel method, not relying on genetic complementation of a mutation, was used to clone a gene for translational initiation factor IF2. Two clones from a cosmid library of total Escherichia coli DNA were isolated for their ability to overproduce IF2 in vivo as determined by quantitative immunoblotti...

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Bibliografiset tiedot
Julkaisussa:Proc Natl Acad Sci U S A
Päätekijät: Plumbridge, J A, Howe, J G, Springer, M, Touati-Schwartz, D, Hershey, J W, Grunberg-Manago, M
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 1982
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6214789/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.16.5033
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