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Preparation of recombinant six-histidine-tagged human LECT2, a chemotactic protein to neutrophils, in Escherichia coli

LECT2 is a chemotactic protein to neutrophils. A recombinant six-histidine-tagged human LECT2, (His)(6)-LECT2, was expressed in E. coli using a pET21a(+) vector. The (His)(6)-LECT2 was purified from the soluble fraction in E. coli as a single band in sodium dodesyl sulfate/polyacrylamide gel electro...

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מידע ביבליוגרפי
Main Authors: Ito, Mie, Yamagoe, Satoshi, Tomizawa, Kazuo, Mizuno, Satoshi, Tanokura, Masaru, Suzuki, Kazuo
פורמט: Artigo
שפה:Inglês
יצא לאור: Kluwer Academic Publishers 1997
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3466745/
https://ncbi.nlm.nih.gov/pubmed/22358898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007976103088
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