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Preparation of recombinant six-histidine-tagged human LECT2, a chemotactic protein to neutrophils, in Escherichia coli
LECT2 is a chemotactic protein to neutrophils. A recombinant six-histidine-tagged human LECT2, (His)(6)-LECT2, was expressed in E. coli using a pET21a(+) vector. The (His)(6)-LECT2 was purified from the soluble fraction in E. coli as a single band in sodium dodesyl sulfate/polyacrylamide gel electro...
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Main Authors: | , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Kluwer Academic Publishers
1997
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3466745/ https://ncbi.nlm.nih.gov/pubmed/22358898 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007976103088 |
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