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Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates

BACKGROUND: While being a standard powerful molecular biology technique, applications of the PCR to the amplification of high GC-rich DNA samples still present challenges which include limited yield and poor specificity of the reaction. Organic solvents, including DMSO and formamide, have been often...

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Detalhes bibliográficos
Main Authors: Farell, Eric M, Alexandre, Gladys
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3466135/
https://ncbi.nlm.nih.gov/pubmed/22624992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-257
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