An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments.

We have developed a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) containing a 500-fold amplification of a 135-kilobase chromosomal DNA sequence. This sequence includes the gene for dihydrofolate reductase (tetrahydrofolate dehydrogenase, 5,6,7,8-tetrahydrofolate: NADP+ oxidoredu...

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Bibliographic Details

Published in:	Proc Natl Acad Sci U S A
Main Authors:	Heintz, N H, Hamlin, J L
Format:	Artigo
Language:	Inglês
Published:	National Academy of Sciences 1982
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346581/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6955792/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.13.4083
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