Marking the polarity of RNA molecules for electron microscopy by covalent attachment of psoralen-DNA restriction fragments.
We have developed a general procedure for covalently attaching complementary restriction fragments to RNA molecules and visualizing the fragment in the electron microscope after denaturation. This is accomplished by creating psoralen monoadducts on the restriction fragment by irradiation at 390 nm b...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1982
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346551/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6287454/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.13.3940 |
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