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A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach
BACKGROUND: The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used meth...
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| Главные авторы: | , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Public Library of Science
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3465266/ https://ncbi.nlm.nih.gov/pubmed/23071657 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0046890 |
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