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Monitoring Shifts in the Conformation Equilibrium of the Membrane Protein Cytochrome P450 Reductase (POR) in Nanodiscs

Nanodiscs are self-assembled ∼50-nm(2) patches of lipid bilayers stabilized by amphipathic belt proteins. We demonstrate that a well ordered dense film of nanodiscs serves for non-destructive, label-free studies of isolated membrane proteins in a native like environment using neutron reflectometry (...

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Библиографические подробности
Главные авторы: Wadsäter, Maria, Laursen, Tomas, Singha, Aparajita, Hatzakis, Nikos S., Stamou, Dimitrios, Barker, Robert, Mortensen, Kell, Feidenhans'l, Robert, Møller, Birger Lindberg, Cárdenas, Marité
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Biochemistry and Molecular Biology 2012
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3464565/
https://ncbi.nlm.nih.gov/pubmed/22891242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.400085
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