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A simple and rapid reverse transcriptase assay for the detection of retroviruses in cell cultures
Reverse transcriptase (RT) is a good diagnostic tool for the detection of retroviruses. We have developed a simple and rapid assay for RT activity in culture supernatants. A 370-base RNA sequence from the tetracycline-resistance gene in pBR322 plasmid DNA was used as a template for RT-mediated cDNA...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Kluwer Academic Publishers
1999
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3463392/ https://ncbi.nlm.nih.gov/pubmed/19003345 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008000210125 |
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