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A simple and rapid reverse transcriptase assay for the detection of retroviruses in cell cultures

Reverse transcriptase (RT) is a good diagnostic tool for the detection of retroviruses. We have developed a simple and rapid assay for RT activity in culture supernatants. A 370-base RNA sequence from the tetracycline-resistance gene in pBR322 plasmid DNA was used as a template for RT-mediated cDNA...

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Detaylı Bibliyografya
Asıl Yazarlar: Kuno, Haruhiko, Ikeda, Hiromi, Takeuchi, Masao, Yoshida, Touho
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Kluwer Academic Publishers 1999
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3463392/
https://ncbi.nlm.nih.gov/pubmed/19003345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008000210125
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