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Purification of two calcium/calmodulin-dependent forms of cyclic nucleotide phosphodiesterase by using conformation-specific monoclonal antibody chromatography

A procedure for nondenaturing immunopurification of bovine calmodulin-dependent 3′,5′-cyclic-nucleotide phosphodiesterase (3′,5′-cyclic-nucleotide 5′-nucleotidohydrolase, EC 3.1.4.17) is described that utilizes chromatography on a conformation-specific monoclonal antibody column. Hybridomas derived...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Hansen, R. Scott, Beavo, J. A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1982
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346291/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6283544/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.9.2788
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