Purification of two calcium/calmodulin-dependent forms of cyclic nucleotide phosphodiesterase by using conformation-specific monoclonal antibody chromatography
A procedure for nondenaturing immunopurification of bovine calmodulin-dependent 3′,5′-cyclic-nucleotide phosphodiesterase (3′,5′-cyclic-nucleotide 5′-nucleotidohydrolase, EC 3.1.4.17) is described that utilizes chromatography on a conformation-specific monoclonal antibody column. Hybridomas derived...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1982
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346291/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6283544/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.9.2788 |
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