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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Förster resonance energy transfer (FRET)-based reporters(1) allow the assessment of endogenous kinase and phosphatase activities in living cells. Such probes typically consist of variants of CFP and YFP, intervened by a phosphorylatable sequence and a phospho-binding domain. Upon phosphorylation, th...

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Bibliografski detalji
Glavni autori: Hukasova, Elvira, Silva Cascales, Helena, Kumar, Shravan R., Lindqvist, Arne
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3462574/
https://ncbi.nlm.nih.gov/pubmed/22314640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3410
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