Improving FRET dynamic range with bright green and red fluorescent proteins

A variety of genetically encoded reporters use changes in fluorescence (or Förster) resonance energy transfer (FRET) to report on biochemical processes in living cells. The standard genetically encoded FRET pair consists of cyan and yellow fluorescent proteins (CFP and YFP), but many CFP-YFP reporte...

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Bibliografiske detaljer
Main Authors: Lam, Amy, St-Pierre, François, Gong, Yiyang, Marshall, Jesse D., Cranfill, Paula J., Baird, Michelle A., McKeown, Michael R., Wiedenmann, Jörg, Davidson, Michael W., Schnitzer, Mark J., Tsien, Roger Y., Lin, Michael Z.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3461113/
https://ncbi.nlm.nih.gov/pubmed/22961245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2171
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