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Improving the photostability of bright monomeric orange and red fluorescent proteins

All organic fluorophores undergo irreversible photobleaching during prolonged illumination. While fluorescent proteins typically bleach at a substantially slower rate than many small molecule dyes, lack of photostability remains an important limiting factor for experiments requiring large numbers of...

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Detalhes bibliográficos
Main Authors: Shaner, Nathan C., Lin, Michael Z., McKeown, Michael R., Steinbach, Paul A., Hazelwood, Kristin L., Davidson, Michael W., Tsien, Roger Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2853173/
https://ncbi.nlm.nih.gov/pubmed/18454154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1209
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