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Partial and Full PCR-Based Reverse Genetics Strategy for Influenza Viruses

Since 1999, plasmid-based reverse genetics (RG) systems have revolutionized the way influenza viruses are studied. However, it is not unusual to encounter cloning difficulties for one or more influenza genes while attempting to recover virus de novo. To overcome some of these shortcomings we sought...

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Autores principales: Chen, Hongjun, Ye, Jianqiang, Xu, Kemin, Angel, Matthew, Shao, Hongxia, Ferrero, Andrea, Sutton, Troy, Perez, Daniel R.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3460856/
https://ncbi.nlm.nih.gov/pubmed/23029501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0046378
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