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In vitro binding of gibberellin A(4) to extracts of cucumber measured by using DEAE-cellulose filters

A rapid method for assaying [(3)H]gibberellin A(4) bound to a soluble protein from cucumber hypocotyls by using DEAE-cellulose filter discs is described. The binding is saturable, reversible with unlabeled gibberellin A(4), and has a half-life of association under nonequilibrium conditions at 0-4°C...

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Autors principals: Keith, B., Brown, S., Srivastava, L. M.
Format: Artigo
Idioma:Inglês
Publicat: 1982
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC346005/
https://ncbi.nlm.nih.gov/pubmed/16578759
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