Quantitative ER ↔ Golgi Transport Kinetics and Protein Separation upon Golgi Exit Revealed by Vesicular Integral Membrane Protein 36 Dynamics in Live Cells

To quantitatively investigate the trafficking of the transmembrane lectin VIP36 and its relation to cargo-containing transport carriers (TCs), we analyzed a C-terminal fluorescent-protein (FP) fusion, VIP36-SP-FP. When expressed at moderate levels, VIP36-SP-FP localized to the endoplasmic reticulum,...

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Main Authors: Dahm, Thorsten, White, Jamie, Grill, Stephan, Füllekrug, Joachim, Stelzer, Ernst H.K.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Cell Biology 2001
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC34599/
https://ncbi.nlm.nih.gov/pubmed/11359937
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