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Applying a Real-Time PCR Assay for Histoplasma capsulatum to Clinically Relevant Formalin-Fixed Paraffin-Embedded Human Tissue

A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diag...

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Detalhes bibliográficos
Main Authors: Koepsell, Scott A., Hinrichs, Steven H., Iwen, Peter C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3457455/
https://ncbi.nlm.nih.gov/pubmed/22855519
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01705-12
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