Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.
We present an approach for determining the in vivo distribution of a protein on specific segments of chromosomal DNA. First, proteins are joined covalently to DNA by irradiating intact cells with UV light. Second, these cells are disrupted in detergent, and a specific protein is immunoprecipitated f...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345570/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6379641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.14.4275 |
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