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Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.

We present an approach for determining the in vivo distribution of a protein on specific segments of chromosomal DNA. First, proteins are joined covalently to DNA by irradiating intact cells with UV light. Second, these cells are disrupted in detergent, and a specific protein is immunoprecipitated f...

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Bibliografiske detaljer
Main Authors: Gilmour, D S, Lis, J T
Format: Artigo
Sprog:Inglês
Udgivet: 1984
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC345570/
https://ncbi.nlm.nih.gov/pubmed/6379641
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