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Protoplast formation and regeneration in Lactobacillus delbrueckii

Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa an...

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Bibliografiske detaljer
Main Authors: Singhvi, Mamta, Joshi, Dipti, Gaikaiwari, Shalaka, Gokhale, Digambar V.
Format: Artigo
Sprog:Inglês
Udgivet: Springer-Verlag 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3450288/
https://ncbi.nlm.nih.gov/pubmed/23100814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-010-0008-y
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