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Protoplast formation and regeneration in Lactobacillus delbrueckii
Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa an...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Springer-Verlag
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3450288/ https://ncbi.nlm.nih.gov/pubmed/23100814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-010-0008-y |
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