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Purification of a hyperactive nitrile hydratase from resting cells of Rhodococcus rhodochrous PA-34
A propionitrile-induced nitrile hydratase (NHase), a promising biocatalyst for synthesis of organic amides has been purified from cell-free extract of Rhodococcus rhodochrous PA-34. About 11-fold purification of NHase was achieved with 52% yield. The SDS-PAGE of the purified enzyme revealed that it...
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer-Verlag
2009
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3450019/ https://ncbi.nlm.nih.gov/pubmed/23100776 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-009-0033-x |
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