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Cloning, expression and immunogenicity of ferric enterobactin binding protein Fep B from Escherichia coli O157:H7
The gene coding for ferric enterobactin binding protein from E. coli O157:H7 was amplifi ed. This gene was cloned and expressed as C-terminal His (6)-tagged protein. The SDS-PAGE analysis of the total protein revealed only two distinct bands, with molecular masses of 31kDa and 34kDa. The Ni-NTA chro...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Springer-Verlag
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3450013/ https://ncbi.nlm.nih.gov/pubmed/23100780 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-009-0044-7 |
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