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Transient gene expression in mammalian cells grown in serum-free suspension culture

In order to establish a simple and scaleable transfection system we have used the cationic polymer polyethylenimine (PEI) to study transient transfection in HEK293 and 293(EBNA) cells grown in serum-free suspension culture. The transfection complexes were made directly within the cell culture by con...

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Bibliografiske detaljer
Main Authors: Schlaeger, Ernst-Jürgen, Christensen, Klaus
Format: Artigo
Sprog:Inglês
Udgivet: Kluwer Academic Publishers 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449952/
https://ncbi.nlm.nih.gov/pubmed/19003357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008000327766
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