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Transient gene expression in mammalian cells grown in serum-free suspension culture
In order to establish a simple and scaleable transfection system we have used the cationic polymer polyethylenimine (PEI) to study transient transfection in HEK293 and 293(EBNA) cells grown in serum-free suspension culture. The transfection complexes were made directly within the cell culture by con...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Kluwer Academic Publishers
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449952/ https://ncbi.nlm.nih.gov/pubmed/19003357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008000327766 |
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