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Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering
The present work describes the genetic modification of a hybridoma cell line with the aim to change its metabolic behaviour, particularly reducing the amounts of ammonia and lactate produced by the cells. The cellular excretion of ammonia was eliminated by transfection of a cloned glutamine syntheta...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Kluwer Academic Publishers
1999
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449941/ https://ncbi.nlm.nih.gov/pubmed/19003358 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008012518961 |
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