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Improvement of a cytokine (TNF-α) bioassay by serum-free target cell (WEHI 164) cultivation
The elaboration of a sensitive bioassay for assessment of tumour necrosis factor alpha (TNF-α) in a defined medium is described. The assay is based on the cytotoxic effect of TNF-α on a target cell line, the murine fibrosarcoma WEHI 164 clone 13. Cytotoxicity was assessed by detecting the rate of te...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Kluwer Academic Publishers
1999
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449908/ https://ncbi.nlm.nih.gov/pubmed/22359061 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008052307547 |
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