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Monitoring proteolysis of recombinant human interferon-γ during batch culture of Chinese hamster ovary cells
Proteolytic cleavage of recombinant human interferon-γ (IFN-γ) expressed in Chinese hamster ovary (CHO) cells during batch fermentation has been monitored by mass spectrometric peptide mapping. IFN-γ was purified from cell-free culture supernatant by immunoaffinity chromatography and cleaved by endo...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Kluwer Academic Publishers
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449879/ https://ncbi.nlm.nih.gov/pubmed/9094218 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007947130709 |
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