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Stable expression of recombinant human coagulation factor XIII in protein-free suspension culture of Chinese hamster ovary cells
The recombinant a and bsubunits for human coagulation factor XIII were transfected into Chinese hamster ovary (CHO) cells. CHO cells were amplified and selected with methotrexate in adherent cultures containing serum, and CHO 1-62 cells were later selected in protein-free medium. To develop a recomb...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Kluwer Academic Publishers
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449789/ https://ncbi.nlm.nih.gov/pubmed/19002921 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020555918441 |
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