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A regulatable selective system facilitates isolation of heterologous protein hyper-producing mammalian cells without gene amplification

In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressu...

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Autores principales: Teruya, Kiichiro, Zhang, Yingpei, Katakura, Yoshinori, Shirahata, Sanetaka
Formato: Artigo
Lenguaje:Inglês
Publicado: Kluwer Academic Publishers 2002
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449528/
https://ncbi.nlm.nih.gov/pubmed/19003100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1023945517446
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