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A regulatable selective system facilitates isolation of heterologous protein hyper-producing mammalian cells without gene amplification
In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressu...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Kluwer Academic Publishers
2002
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449528/ https://ncbi.nlm.nih.gov/pubmed/19003100 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1023945517446 |
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