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An enhanced approach for engineering thermally stable proteins using yeast display
Many biotechnology applications require the evolution of enhanced protein stability. Using polymerase chain reaction-based recovery of engineered clones during the screen enrichment phase, we describe a yeast display method capable of yielding engineered proteins having thermal stability that substa...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449400/ https://ncbi.nlm.nih.gov/pubmed/22767886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzs041 |
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