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An enhanced approach for engineering thermally stable proteins using yeast display

Many biotechnology applications require the evolution of enhanced protein stability. Using polymerase chain reaction-based recovery of engineered clones during the screen enrichment phase, we describe a yeast display method capable of yielding engineered proteins having thermal stability that substa...

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Bibliografske podrobnosti
Main Authors: Pavoor, Tej V., Wheasler, Jean A., Kamat, Viraj, Shusta, Eric V.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449400/
https://ncbi.nlm.nih.gov/pubmed/22767886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzs041
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