Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.

The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and (iv) substrata of liv...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Enat, R, Jefferson, D M, Ruiz-Opazo, N, Gatmaitan, Z, Leinwand, L A, Reid, L M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1984
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344845/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6584889/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.5.1411
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