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Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.

The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and (iv) substrata of liv...

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Detalhes bibliográficos
Main Authors: Enat, R, Jefferson, D M, Ruiz-Opazo, N, Gatmaitan, Z, Leinwand, L A, Reid, L M
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC344845/
https://ncbi.nlm.nih.gov/pubmed/6584889
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