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Solution NMR of a 463-Residue Phosphohexomutase: Domain 4 Mobility, Substates, and Phosphoryl Transfer Defect

Phosphomannomutase/phosphoglucomutase contributes to infectivity of Pseudomonas aeruginosa, retains and reorients its intermediate by 180°, and rotates domain 4 to close the deep catalytic cleft. NMR spectra of the backbone of wild-type and S108C-inactivated enzymes were assigned to at least 90%. (1...

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Autors principals: Sarma, Akella V.S., Anbanandam, Asokan, Kelm, Allek, Mehra-Chaudhary, Ritcha, Wei, Yirui, Qin, Peiwu, Lee, Yingying, Berjanskii, Mark V., Mick, Jacob A., Beamer, Lesa J., Van Doren, Steven R.
Format: Artigo
Idioma:Inglês
Publicat: 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3448444/
https://ncbi.nlm.nih.gov/pubmed/22242625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201609n
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