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Contact points between transcription machinery and the fibroin gene promoter deduced by functional tests of single-base substitution mutants.

An efficient method for oligonucleotide-directed mutagenesis was developed to construct a set of site-specific mutations by using a mixture of oligodeoxyribonucleotides. With this method, as high as 40% of the tested clones turned out to be desired mutants. Seven single-point mutants were isolated i...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Hirose, S, Takeuchi, K, Hori, H, Hirose, T, Inayama, S, Suzuki, Y
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 1984
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344841/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6369326/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.5.1394
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