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Contact points between transcription machinery and the fibroin gene promoter deduced by functional tests of single-base substitution mutants.
An efficient method for oligonucleotide-directed mutagenesis was developed to construct a set of site-specific mutations by using a mixture of oligodeoxyribonucleotides. With this method, as high as 40% of the tested clones turned out to be desired mutants. Seven single-point mutants were isolated i...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344841/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6369326/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.5.1394 |
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