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A Facile Method to Synthesize Histones with Posttranslational Modification Mimics
Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3448024/ https://ncbi.nlm.nih.gov/pubmed/22697363 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300535a |
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