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A Facile Method to Synthesize Histones with Posttranslational Modification Mimics

Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles...

詳細記述

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書誌詳細
主要な著者: Wang, Zhiyong U., Wang, Yane-Shih, Pai, Pei-Jing, Russell, William K., Russell, David H., Liu, Wenshe R.
フォーマット: Artigo
言語:Inglês
出版事項: 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3448024/
https://ncbi.nlm.nih.gov/pubmed/22697363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300535a
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