Molecular cloning of hormone-responsive genes from the yeast Saccharomyces cerevisiae.
A method for identifying yeast genes whose transcription is differentially regulated was developed. The technique is based on incorporation of the analog 4-thiouridine into nascent RNAs, which allows their purification. The purified RNAs are used to prepare cDNA copies for screening of genomic DNA l...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1984
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344782/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6366798/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.4.1144 |
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